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List of Post-Doctoral Fellowships Junior Fund

As part of Charles University’s constant efforts to develop and strengthen its research base we are keen to recruit high-quality post-doctoral research fellows from abroad to work at top University or faculty centres and contribute to the University’s strong research profile. More information on the Junior Fund here can be found here.

Below is a list of research projects announced in 2021 (positions available from January 2022):

Title of the research project: Biomimetic remineralization and hard tissue biomodulation

Input Premise: As a consequence of the age-related gingival recession and the frequent exposure of cervical root dentin, the risk of root dentin caries has increased. However, root cavities may be difficult to treat because of the complex etiology and structure. Biomimetic remineralization could be a powerful approach for the treatment of such defects. Specifically, self-assembling peptides (SAPs) are increasingly gaining interest for potential use as scaffolds in tissue engineering [1]. As previous studies reported that cross-linking agents such as proanthocyanidin (PA) reinforced the properties of dentin collagen matrix and improved the bond strength of adhesives to dentin [2], PA could also reinforce the scaffold self-assembled within the body of a subsurface lesion. The remineralization of the scaffold would be stimulated by bioactive glass particles (BAGs) or casein phosphopeptides-amorphous calcium phosphate (CPP-ACP). BAGs are highly biocompatible materials with a wide variety of use in medical and dental fields because of their ability to support the growth of bone cells and formation of hydroxyapatite (Fig. 2) [3]. CPP-ACP is a bioactive material used to initiate and promote the remineralization of enamel and dentin structures as CPP has the ability to stabilize calcium phosphate in solution by binding the ACP with phophoserine residues, leading to formation of nano CPP-ACP clusters [4].

This project is aimed at evaluating the potential of SAPs combined with BAGs or CPP-ACP to induce biomimetic remineralization of enamel, dentin and bone tissues. The properties of the remineralized tissues would be assessed using various methods including micro-computed tomography, scanning electron microscopy, transmission electron microscopy, microhardness, FTIR spectroscopy, and X-ray diffraction. It is expected that the biomechanical properties of the remineralized tissues and hence the clinical outcome of the treatment would be enhanced if biomimetic remineralization is achieved.

Required Qualification:

· Ph.D. degree in dental medicine, life sciences, or related fields (max. 5 years from graduation)

· Record of publications in IF journals: at least 3 publications in IF journals (IF above 1.5), at least one as a first author

· Ability to communicate in both spoken and written English (minimum level B2 in the Common European Framework of Reference or equivalent)

· High motivation, ability to conduct collaborative research.

Workplace/: Institute of Dental Medicine (First Faculty of Medicine of the Charles University and General University Hospital in Prague)

Supervisor: Dr. Antonin Tichy, PhD

E-mail of the supervisor: antonin.tichy@lf1.cuni.cz

Phone of the supervisor: +420 224 96 68 05

Position available from: January 1, 2022

Deadline date for applications: July 18, 2021

Applicants must submit required documents to: Anna Jezberováanna.jezberova@lf1.cuni.cz


Title of the research project: Physiopathology of T-type calcium channels in motor neuron function

Input Premise: Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by the progressive loss of cortical, brain stem, and spinal motor neurons that leads to muscle weakness and death. ALS is regarded as a complex genetic disorder with a Mendelian pattern of inheritance in 5-10% of cases (familial ALS), but most patients have no discernable family history of the disease (sporadic ALS). However, several genes in apparent sporadic ALS are believed to increase the risk and/or modify the onset/progression of the disease. Recent studies from our laboratory suggest that CACNA1H encoding for Cav3.2 T-type Ca2+ channels may represent a risk factor for the disease. The goal of this research proposal is to address clinically relevant, fundamental questions regarding the role of T-type Ca2+ channels in motor neuron function, with a key translational aim of elucidating their pathogenic role in the development of motor neuron disorders such as (ALS).

Work environment:

The candidate will benefit from modern instrumentation including a patch clamp electrophysiology, confocal microscopy, efficient animal and cell culture facilities, as well as all the necessary equipment for regular molecular biology and biochemistry. More information can be found on our lab webpage http://theweisslab.com

Required Qualification:

The candidate must have a Ph.D or equivalent degree in neuroscience, cell biology or equivalent. Prior experience with patch clamp electrophysiology will be appreciated. Experience with primary neuronal cell culture, basic molecular (PCR, mutagenesis) and biochemistry (western blot) techniques, or confocal imaging microscopy will be an added advantage.

Workplace: Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University

Supervisor: Dr. Norbert Weiss, Ph.D (nalweiss@gmail.com)

Position available from: January 1, 2022

How to apply: If you are interested in this position, please send a short cover letter describing your scientific interests along with a CV directly to Dr. Norbert Weiss nalweiss@gmail.com no later than June 30, 2021 to discuss and prepare your application

Deadline date for applications: July 18, 2021

Applicants must submit required documents to: Anna Jezberováanna.jezberova@lf1.cuni.cz



Title of the research project: DNA distribution in the cell nucleus - a multidisciplinary imaging approach

Input Premise: The cell nucleus is probably the most complex compartment of the cell. It contains the genome and is the site of all related activities such as DNA replication and repair, RNA synthesis, as well as RNA maturation and transport. These activities take place within dynamic three-dimensional non-membrane domains. A comprehensive structural-functional study of these domains requires an approach integrating state-of-the-art in situ imaging methods at various levels of resolution, and a combination of in vivo analysis with a subsequent ultrastructural investigation performed on the same cells.

The major aim of the proposed project is an analysis of the DNA distribution within the nuclear volume including a reconstruction of the large scale three-dimensional (3D) chromatin arrangement in mammalian cell nuclei. This will shed light on the longstanding controversies about chromatin architecture in the interphase nucleus.

This project will be pursued with synchronised cell lines. To visualise transcriptionally active chromatin, the cells will be labelled by in vivo incorporation of uridine marked with halogens and/or stable isotope 15N or 13C. Heavy-metal stains that selectively enhance the contrast of chromatin will be used. For structure and function analysis, transmission and scanning electron microscopy (TEM and SEM) approaches (SEM array tomography and combination of SEM with focused ion-beam milling) together with the nanoscale secondary ion mass spectrometry (NanoSIMS) will be applied.

Workplace: Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University and General University Hospital in Prague

Supervisor: Dusan Cmarko, Ph.D.

E-mail: dusan.cmarko@lf1.cuni.cz

Position available from: January 1, 2021

Deadline date for applications: July 18, 2021

Applicants must submit required documents to: Anna Jezberováanna.jezberova@lf1.cuni.cz



Title of the research project: Genetics and Mechanisms of Amyotrophic Lateral Sclerosis (ALS)

Amyotrophic lateral sclerosis is the third most common neurodegenerative disorder after Parkinson`s and Alzheimer`s. ALS is a devastating disorder affecting people in their 40–60 years of age, with an average survival of 2–3 years. There is no cure. Genetic defects have been found in both, familial and sporadic ALS, and twin studies estimate the heritability to about 60 %. Mutations causing ALS leads to various biological consequences disturbing pathways of cellular energy metabolism, protein homeostasis, RNA metabolism, neuroinflammation, cytoskeletal pathways and many others. The diversity of genetic architecture and clinical phenotypes challenges the ultimate goal to find a cure. Up today, defects in more than 30 genes have been identified to cause ALS, including c9orf72, TARDBP, SOD1, FUS, ATXN2, TBK1, and other. Also about 20 genes contribute to disease development as risk factors. Therefore, current drug development focuses on numerous targets, but for clinical trials we must also cluster patients according to their phenotypes. Therefore, we want to characterize the genotype and phenotype in our cohort of patients with sporadic and familial ALS. We have selected Slavic population from central Europe, characterized by common lifestyle and similar epidemiology. For phenotyping, we will collaborate with Neuromuscular centers to obtain clinical data and questionnaires.

This project aims to

1. design and to build a database for data describing ALS phenotypes

2. analyze genetic and genomic data of Slavic population (NGS, GWAS) and to compare the results with the current studies

3. identify the risk factors affecting the disease or its course

To collect and to analyze the data describing ALS phenotype, we will build secured database, which will be used for further analyses. As inclusion criteria, only patients with certain ALS diagnosis confirmed by a neurologist will be included. General epidemiologic data will be collected as age, sex, occupation, as well as data specific to ALS: the age of onset, site of onset of the disease, contact sports, attendance in military service, ALSFRS-R (a scale describing disease progression).

Results from genetic/genomic analyses from Next Generation Sequencing or Genotyping Arrays (Illumina) will be analyzed with R software.

This is a collaborative work with neuromuscular centers from university hospitals, state-of the-art facilities (Charles University, BIOCEV), and bioinformatic structures. This work is supported by PRIMUS 21/MED/012 and other funding.

Workplace: Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University in Prague

Supervisor: Lenka Šlachtová, PhD.

E-mail: lenka.slachtova@lf1.cuni.cz

Position available from: January 1, 2021

Deadline date for applications: July 18, 2021

Applicants must submit required documents to: Anna Jezberováanna.jezberova@lf1.cuni.cz



Hodnocení: spravovat